1. Prepare the CD cases for the seeds to be placed in. Fold the paper towel to fit into the transparent CD cases.

2. Place the cut paper towels in six individual CD cases.

3. Moisten the paper towel. It should not be dry or dripping wet.

4. Place five seeds in the middle of the moist paper, roughly evenly spaced from each other. None of the seeds should be close to the sides of the paper. Amount of seeds will be made standard among each CD case to maintain accuracy of the result.

5. Close the CD cases. Repeat the steps above for the 5 other CD cases.

6. Use Blue-Tacks to hold two sets of CD cases (labeled #1 and #2) upright, with the label ‘Up’ on the top edge in their individual Petri Dishes. Selected roots of one of the seeds that are perpendicular to the base of the Petri dish in CD cases one and two will be the control for the experiment.

7. Place the other two CD cases (labeled #3 and #4) horizontally (flat) in two more individual Petri dishes.

8. Set the final two CD cases (labeled #5 and #6) vertically secured with Blue-Tack on two more Petri dishes, upright with the label "Up" at the top. These CD cases will be rotated 90 degrees clockwise every 2 days to observe how this change of direction affects the root growth.

9. Place all of the CD cases inside a large, lightproof cardboard box where they will not be disturbed by light.

10. Check the CD cases once a day. Keep the seeds moist by carefully opening the CD cases and using the 10ml syringe to slowly and carefully add a little water to each CD case. Caution must be taken not to dislodge the seeds. Close and return the CD cases to the lightproof box after this is completed.

11. Measure the incident angle using a protractor and a ruler, in order to find the change in angle in the roots with respect to the control. Record the angles of the root growth for each seed daily in the logbook for 5 days

12. Repeat the experiment ( steps 1-11) again for another 5 days to obtain more accurate data.

13. After repeating the experiment, find the average of the growth and angle of the growth of seeds for both experiments then compare and find the average between then to have a more accurate result.

## No comments:

## Post a Comment